UDP-glucose pyrophosphorylase (UDP-glucose pyrophosphosphprylase, UGP, EC2.7.7.9) is widely
distributed in nature. It catalyzes the activation of glucose before glycogen synthesis. UDP-glucose
(UDPG) is synthesized from glucose-1-phosphate and UTP. UDPG is the main active enzyme form in
higher plants and animals. As a glucose-based donor, it participates in the synthesis and metabolism of
glycogen, sucrose, cellulose, etc.
UGP can catalyze the reversible formation of glucose-1-phosphate. NADP was transformed into NADPH
by phosphoglucose mutase and 6-phosphoglucose dehydrogenase. UGP activity can be reflected by the
change of 340nm absorption value.
Required but Not Provided:
Ultraviolet spectrophotometer, balance, low temperature desk centrifuge, water-bath, transferpettor, 1 mL
quartz cuvette, EP tube, mortar/homogenizer, ice and distilled water.
Protocol
I. Preparation: