UDP-glucose pyrophosphosphprylase(UGP) Activity Assay Kit

Home > Product > Test Kit > UDP-glucose pyrophosphosphprylase(UGP) Activity Assay Kit

UDPG焦磷酸化酶（UGP）活性检测试剂盒

Cat:

NA0388

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: Liquid 110 mL×1, store at 4℃;

Reagent I: Powder×1, store at -20℃ and protect from light; Add 15 mL distilled water to fully dissolve

before use. The remaining reagents can be stored for two weeks at -20℃. Do not freeze and thaw

repeatedly.

Reagent II: Powder×1, store at 4℃ and protect from light; Add 2.5 mL distilled water to fully dissolve

before use. The remaining reagents can be stored for one week at 4℃. Do not freeze and thaw repeatedly.

Reagent III: Powder×1, store at -20℃ and protect from light; Add 1.4 mL distilled water to fully dissolve

before use. The remaining reagents can be stored for two weeks at -20℃. Do not freeze and thaw

repeatedly.

Reagent IV: Powder×1, store at -20℃ and protect from light; Add 1 mL distilled water to fully dissolve

before use. The remaining reagents can be stored for two weeks at -20℃. Do not freeze and thaw

repeatedly.

Reagent V: Liquid 2.5 mL×1, store at 4℃;

Reagent VI: Liquid 5 mL×1, store at 4℃;

prev next

Unit:

Price: $1617

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
Feedback Wall

UDP-glucose pyrophosphorylase (UDP-glucose pyrophosphosphprylase, UGP, EC2.7.7.9) is widely

distributed in nature. It catalyzes the activation of glucose before glycogen synthesis. UDP-glucose

(UDPG) is synthesized from glucose-1-phosphate and UTP. UDPG is the main active enzyme form in

higher plants and animals. As a glucose-based donor, it participates in the synthesis and metabolism of

glycogen, sucrose, cellulose, etc.

UGP can catalyze the reversible formation of glucose-1-phosphate. NADP was transformed into NADPH

by phosphoglucose mutase and 6-phosphoglucose dehydrogenase. UGP activity can be reflected by the

change of 340nm absorption value.

Required but Not Provided:

Ultraviolet spectrophotometer/microplate reader, balance, low temperature desk centrifuge, water-bath,

transferpettor, micro quartz cuvette/96-well flat-bottom UV plate, EP tube, mortar/homogenizer, ice and

distilled water.

Protocol

I. Preparation:

1. Tissue: according to the ratio of mass (g): extraction volume (mL): 1:5-10 to add the extract. It is

suggested that add 1 mL of extract to 0.1 g of tissue. Homogenate on ice. Centrifuge at 10000 g 4℃ for 10

min. Take the supernatant on ice for test.

2. Bacteria and cells: according to the ratio of 10⁴cells: extract volume (mL) 500-1000:1. It is suggested to

take about 500 million bacteria/cells and add 1 mL extraction reagent. Bacteria/cells is split by

ultrasonication (power 300w, ultrasonic 3s, interval 7s, total time 3 min). Centrifuge at 10000 g 4℃ for 10

min. Take the supernatant on ice for test.

3. Serum and other liquids: detect directly.

II. Determination

Product Feedback Wall