H2O2 is the most common reactive oxygen molecules in organisms. It is mainly produced by the
catalyzation of SOD and XOD and degraded by the catalyzation of CAT and POD. H2O2, which is not only
one of the important reactive oxygen, but also the hub of mutual conversion of reactive oxygen. On the one
hand, H2O2 can directly or indirectly oxidize intracellular nucleic acids, proteins and other biological
macromolecules, and damage cell membranes, thus accelerating the aging and disintegration of cells. On
the other hand, H2O2 is also a key regulatory factor in many oxidative emergency reactions.
H2O2 and titanium sulfate generate yellow titanium peroxide complex with the characteristic absorption at
415nm.
Reagents and Equipment Required but Not Provided.
Spectrophotometer/microplate reader, desk centrifuge, adjustable pipette, micro glass cuvette/96 well
flat-bottom plate, transferpettor, acetone concentrated sulfuric acid (37% HCl), mortar and ice.
