S-GLS (EC3.5.1.2) exists in some bacteria and plant roots, catalyzes the hydrolysis of glutamine to
glutamic acid and ammonia. It has important regulatory effects on nitrogen metabolism, especially the
regulation of free ammonia content and urea metabolism.
S-GLS catalyzes the hydrolysis of glutamine to L-glutamic acid and ammonia. The rate of ammonia
increase can be calculated by the indophenol blue colorimetric method, and its enzyme activity can be
calculated.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, adjustable transferpettor, balance, mortar/homogenizer, centrifuge, 1mL glass
cuvette, sieve (30-50 mesh, or smaller), toluene, ice and distilled water.
Sample preparation:
Fresh soil samples are naturally air-dried or oven dried at 37℃ and passed sieve (30-50 mesh).
