Soil Lipase(S-LPS) Activity Assay Kit

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土壤脂肪酶（S-LPS）活性检测试剂盒

Cat:

NA0370

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/24S

Storage instructions：

2-8℃

Product Overview：

Components:

Reagent I: 30 mL×1, storage at 4℃.

Reagent II: 6 mL×1, storage at room temperature.

Reagent III: Powder×1, storage at 4℃.

Reagent IV: 20 mL×1, storage at 4℃.

Standard: 59.3 μL×1, storage at 4℃. Before use add 1.435 mL of toluene to obtain 125 μmol/ml oleic acid.

Pay attention to thawing and dissolving before use.

Preparation of working solution: Add 20 mL of distilled water into the Reagent III in the boiling water bath

to dissolve before use, cool it to room temperature, add 5 mL of Reagent II into the solution, mixing, shake

it twice at high speed, 3 minutes of each time, 5 minutes of interval. Store at 4℃. Prepare when the

solution will be used according to the proportion.

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Unit:

Price: $97

Product PDFs

Datasheet:

Lipase (LPS), also known as glyceride hydrolase, catalyzes the hydrolysis of triglycerides to produce fatty

acids and glycerol (or diacylglycerol and monoesters). The enzyme plays an important role in soil

biological dynamics.

LPS catalyzes the hydrolysis of oil esters to fatty acids. The activity of LPS can be calculated by

measuring the rate of fatty acid formation with copper soap method.

Reagents and Equipment Required but Not Provided：

Desktop centrifuge, shaker mixer, spectrophotometer, 1 mL glass cuvette, transferpettor, toluene, ice and

distilled water, 30 mesh sieve (or smaller).

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