Soil aryl sulfatase come from soil microorganism, which can catalyze the transformation of soil organic
sulfide into plant absorbable inorganic sulfur. It plays an important role in the biochemical cycle of sulfur
and sulfur nutrition metabolism of plants, and it is an important biological index reflecting soil quality.
S-ASF can catalyzes the formation of p-nitrophenol from p-nitrobenzenesulfate, which has characteristic
light absorption at 410 nm.
Reagents and Equipment Required but Not Provided.
Spectrophotometer, table centrifuge, water-bath, transferpettor, 1 mL glass cuvette, mortar, ice, 30-50
mesh sieve (or smaller), toluene (express delivery is not allowed) and distilled water.