S-LAP is a kind of enzyme that can hydrolyzes the N-terminal of peptide chain to leucine, which is
secreted by soil microorganism. The changes of S-LAP activity are closely related to some pathological
states.
S-LAP decomposes L-leucine-p-nitroaniline to p-nitroaniline, the latter has the maximum absorption peak
at 405 nm, and the activity of S-LAP is calculated by measuring the high rate of absorption value.
Reagents and Equipment Required but Not Provided.
Scales, centrifuge, spectrophotometer, 1 mL glass cuvette, toluene, acetone, 30 mesh sieve (or smaller).
