Soil β-1,4-Glucanase（S-C1）Activity Assay Kit

Home > Product > Test Kit > Soil β-1,4-Glucanase（S-C1）Activity Assay Kit

土壤β

Cat:

NA0360

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

2-8℃

Product Overview：

Components:

Reagent I: Toluene 2mL×1. Storage at 4℃. (self-provided reagent)

Reagent Ⅱ: Powder×2. Storage at 4℃. Add 7.5 mL of Reagent Ⅲ to fully dissolve when the solution

will be used. Store unused reagents at 4°C.

Reagent Ⅲ: 40 mL×1. Storage at 4℃.

Reagent IV: 30 mL×1. Storage at 4℃.

Standard solution: 1mL×1, 5mmol/L p-nitrophenol solution. The standard is diluted 50 times with reagent

III to obtain a 100μmol/L standard solution before use.

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Unit:

Price: $264

Product PDFs

Datasheet:

β-1,4-glucanase/cellobiosidase (C1, EC3.2.1.91) exists in bacteria, fungi and animals, and is a component

of the cellulase system. The end of the linear molecule hydrolyzes the β-glucosidic bond and cuts out one

cellobiose molecule every time.

S-C1 can catalyze p-nitrobenzene cellobiose (PNPC) to p-nitrophenol, which has a characteristic light

absorption at 400nm.

Reagents and Equipment Required but Not Provided

Spectrophotometer/ Microplate reader, centrifuge, water-bath, transferpettor, micro glass cuvette/ 96-well

flat-bottom plate, mortar, toluene, sieve (30-50 mesh) and distilled water.

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