Home > Product > Test Kit > Soil Neutral Invertase(S-NI) Activity Assay Kit
土壤中性转化酶(S-NI)活性检测试剂盒
Cat:
NA0373
Assay Type:
Spectrophotometer
Brand:
sunlong medical
Specificity:
50T/24S
Storage instructions:
2-8℃
Product Overview:
Components:  
Reagent I: 100 mL×1. Storage at 4.  
Reagent Ⅱ: Powder×1. Storage at 4℃. Add 30 mL of Reagent I to fully dissolve for standby when the  
solution will be used. Store unused reagents at 4.  
Reagent : 30 mL×1. Storage at 4.  
Standard solution: powder×1, 10 mg of anhydrous glucose. Storage at 4℃; Add 1 mL of distilled water  
with filly dissolve before use to prepare 10 mg/mL glucose standard solution for standby.  
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Unit:
Price: $
Product PDFs
Datasheet:


S-NI catalyzes the irreversible decomposition of sucrose into fructose and glucose under neutral  
conditions, and is one of the key enzymes for sucrose metabolism in soil microorganisms.  
S-NI catalyzes the degradation of sucrose to produce reducing sugar, and further reacts with 3,5-  
dinitrosalicylic acid to form brownish red amino compound, which has characteristic light absorption at  
540 nm, and the increase rate of light absorption at 540 nm in a certain range is in direct proportion to NI  
activity. Within a certain range the activity of S-NI is calculated by the increasing rate of light absorption.  
Reagents and Equipment Required but Not Provided  
Spectrophotometer, centrifuge, water-bath, transferpettor, 1 mL glass cuvette, mortar, toluene, sieve (30-  
50 mesh) and distilled water.  
 
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