Anthocyanin reductase (ANR) is a key enzyme in the biosynthesis pathway of procyanidins, which converts
anthocyanins into the cis-flavan-3-alcohol. It plays an important role in plants regulation.
ANR converts cyanidin chloride to flavane-3-alcohol under the action of NADPH. The activity of ANR can
be reflected by measuring the reduction rate of NADPH at 340nm.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, low temperature centrifuge, adjustable transferpettor, water bath, 1ml quartz cuvette,
mortar, ice, alcohol and distilled water.
Sample preparation:
1. Tissue: Add 1 ml of extract solution into 0.1g of tissue, fully grinding on ice. centrifuge at 12000rpm 4
℃ for 15min, supernatant on ice is used for test.
2. Cells or microbial sample: collect cells or microbial sample to centrifuge and remove supernatant.
Suggested 5 million with 1mL of extract solution, split bacteria and cells with ultrasonication (power
20%, work time 3s, interval 10s, for 30 times), centrifuge at 12000rpm 4℃ for 15min, supernatant on
ice is used for test.
