Indoleacetic acid oxidase Activity Assay Kit

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吲哚乙酸氧化酶活性检测试剂盒

Cat:

NA0348

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: 60ml×1 bottle, storage at 4℃.

Reagent I: powder×1 bottle, storage at 4℃, dissolve with 5ml of distilled water before use;

Reagent II: powder×1 bottle, storage at 4℃, dissolve with 3ml of distilled water before use;

Reagent III: powder×1 bottle, storage at -20℃, dissolve with 5.71ml of 50% alcohol (alcohol volume:

water volume=1:1) before use. It can be stored at -20℃ after dispensing to avoid repeated freezing and

thawing.

Reagent IV: 30ml×1 bottle, storage at 4℃.

Reagent V: powder×1 bottle, storage at 4℃, dissolve with 15ml of reagent IV for use;

Standard: powder×1 bottle, 10 mg of indoleacetic acid, storage at -20℃ and avoid light. Add 1.14ml of

50% alcohol (alcohol volume: water volume=1:1) for use to make 50umol/mL standard solution. It can be

stored at -20℃ after dispensing to avoid repeated freezing and thawing.

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Unit:

Price: $213

Product PDFs

Datasheet:

Indoleacetic acid (IAA) is deactivated and damaged under the catalyzation of indoleacetic acid oxidase.

IAA oxidase can regulate the level of indoleacetic acid in plants and affect plant growth.

In the condition of inorganic acid, IAA react with FeCl₃to form red product, which has absorption peak at

530nm.The enzyme activity can be expressed by the rate of destruction of IAA.

Reagents and Equipment Required but Not Provided:

Spectrophotometer/ microplate reader, micro quartz cuvette/96 well flat-bottom UV plate, water bath, low

temperature centrifuge, adjustable transferpettor, mortar, alcohol, ice and distilled water.

Sample preparation:

1. Tissue: Add 1 ml of extract solution into 0.1g of tissue, fully grinding on ice. centrifuge at 12000rpm

and 4℃ for 15 min, supernatant on ice is used for test.

2. Cells or microbial sample: collect cells or microbial sample to centrifuge and remove supernatant.

Suggested 5 million with 1mL of extract solution, split bacteria and cell with ultrasonication (power

20%, work time 3s, interval 10s, for 30 times). Centrifuge at 12000rpm and 4℃ for 15min,

supernatant on ice is used for test.

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