Leucine aminopeptidase (LAP) is a kind of membrane binding enzyme, which widely exists in liver,
gallbladder, pancreas and other tissues. LAP participates in the degradation and renewal of tissue proteins
and some peptides. Because of the damage of liver cells, the activity of serum LAP in patients with various
liver diseases has increased in varying degrees. LAP can be used as a preliminary detection index for various
liver diseases, especially for the differential diagnosis of liver cancer.
LAP decomposes L-leucine-p-nitroaniline to produce p-nitroaniline, the latter has a maximum absorption
peak at 405 nm, and the activity of LAP is calculated by measuring the rising rate of absorption value.
Reagents and Equipment Required but Not Provided:
Scales, low temperature centrifuge, spectrophotometer/microplate reader, micro glass cuvette/96 well flat-
bottom plate, acetone, homogenizer/mortar.
