Proline Dehydrogenase(ProDH) Activity Assay Kit

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脯氨酸脱氢酶（ProDH）活性检测试剂盒

Cat:

NA0340

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution I：100 mL×1. Storage at 4℃.

Extract solution II：1.2 mL×1. Storage at 4℃.

Reagent I: 20 mL×1. Storage at 4℃.

Reagent II: Powder×1. Storage at 4℃. When the solution will be used, add 2.5 mL of distilled water to fully

dissolve for future use, and keep the unfinished reagent at 4℃.

Reagent III: Powder×1. Storage at 4℃. When the solution will be used, add 4 mL of distilled water to fully

dissolve for future use, and keep the unfinished reagent at 4℃.

Reagent IV: Powder×1. Storage at -20℃. When the solution will be used, add 5 mL of distilled water to

fully dissolve for future use, and the unused reagents can be packed separately and stored at -20℃ to avoid

repeated freezing and thawing.

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Unit:

Price: $317

Product PDFs

Datasheet:

Proline Dehydrogenase (ProDH) is a key enzyme that catalyzes proline degradation in mitochondria.

Reducing ProDH activity is very important for regulating osmotic balance, preventing osmotic stress from

damaging plants, scavenging free radicals and protecting cell structure.

ProDH catalyzes the dehydrogenation of proline to pyruvic acid. The dehydrogenation is transferred to

reduce 2,6-dichlorophenol indophenol (DCPIP) by phenazine dimethyl sulfate (PMS), and it has a

characteristic absorption peak at 600 nm. The reduction rate of 2,6-DCPIP is determined by the decrease of

600 nm absorbance, which represents ProDH activity.

Reagents and Equipment Required but Not Provided:

Scales, low temperature centrifuge, spectrophotometer/microplate reader, micro glass cuvette/96 well flat-

bottom plate, acetone, homogenizer/mortar.

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