Cinnamyl alcohol dehydrogenase (CAD) is one of the key enzymes in the lignin biosynthetic pathway,
which catalyzes the formation of cinnamyl alcohol that corresponds to coumarin, mustard aldehyde and
coniferaldehyde. CAD is mostly found in higher plants, yeast, and bacteria, the study of this enzyme can
explore the metabolic mechanism of lignin deposition during the development of various biological cells,
and provide a basis for reducing the content of fruit stone cells and improving their quality.
CAD catalyzes cinnamaldehyde and NADPH to cinnamyl alcohol and NADP+. In this kit, the activity of
CAD is determined by the decrease rate of NADPH at 340 nm.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, desk centrifuge, adjustable transferpettor, water bath, 1 mL quartz cuvette,
mortar/homogenizer, ice, absolute ethanol and distilled water.
