Lignin is one of the components of plant cell wall. It has the function of connecting cells. Lignin exists in
xylem. The main function is to harden cell wall by forming interwoven net, which is the main component
of secondary wall.
There is a characteristic absorption peak at 280 nm after acetylation of phenolic hydroxyl in lignin. The
absorbance value of 280 nm is positively correlated with lignin content.
Reagents and Equipment Required but Not Provided.
Ultraviolet spectrophotometer/microplate reader, table centrifuge, water-bath, micro quartz cuvette/96 well
UV flat-bottom plate (non-polystyrene material), transferpettor, mortar/ homogenizer, EP tube, parafilm,
perchloric acid, glacial acetic acid and distilled water.
