β-1,4-glucanase/cellobiosidase (C1, EC3.2.1.91) exists in bacteria, fungi and animals, and is a component
of the cellulase system. The end of the linear molecule hydrolyzes the β-glucosidic bond and cuts out one
cellobiose molecule every time.
C1 can catalyze p-nitrobenzene cellobiose (PNPC) to p-nitrophenol, which has a characteristic light
absorption at 400nm.
Reagents and Equipment Required but Not Provided
Spectrophotometer, centrifuge, water-bath, transferpettor, 1 mL glass cuvette, mortar/homogenizer, and
distilled water.