1,4-β-D-Glucan Cellobilhydrolase (C1) Activity Assay Kit

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外切β

Cat:

NA0309

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

2-8℃

Product Overview：

Components:

Extract solution:60mL×1. Storage at 4℃.

Reagent I: Powder×1. Storage at 4℃. Add 5 mL of distilled water to fully dissolve when the solution will

be used. Store unused reagents at 4℃ for 2 weeks.

Reagent II:30mL×1. Storage at 4℃.

Standard solution: 1mL×1, 5μmol/mL p-nitrophenol solution. The standard is diluted 16 times with reagent

III to obtain a 0.3125 μmol/mL standard solution before use.

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Unit:

Price: $229

Product PDFs

Datasheet:

β-1,4-glucanase/cellobiosidase (C1, EC3.2.1.91) exists in bacteria, fungi and animals, and is a component

of the cellulase system. The end of the linear molecule hydrolyzes the β-glucosidic bond and cuts out one

cellobiose molecule every time.

C1 can catalyze p-nitrobenzene cellobiose (PNPC) to p-nitrophenol, which has a characteristic light

absorption at 400nm.

Reagents and Equipment Required but Not Provided

Spectrophotometer/ microplate reader, centrifuge, water-bath, transferpettor, micro glass cuvette/ 96 well

flat-bottom plate, mortar/homogenizer, and distilled water.

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