Tissue Iron Content Assay Kit

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组织铁含量检测试剂盒

Cat:

NA0301

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

2-8℃

Product Overview：

Components:

Extract solution: Liquid 110 mL×1. Storage at 4℃.

Reagent I: Powder×2. Storage at 4℃. Add 5 mL of distilled water before use. Prepare the reagent when it

will be used. When the reagent turns black, it cannot be used;

Reagent Ⅱ: Powder×2. Storage at 4℃. Add 235μL of glacial acetic acid and 7.5 mL of distilled water

before use. Unused reagent can be stored for one week at 4 ℃.

Standard Solution: Liquid 3 mL×1, 1μmol/mL Fe³⁺standard solution. Storage at 4℃. Add distilled water

dilute 8 times to form a standard solution of 0.125μmol/mL before use. Prepare when the solution will be

used.

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Unit:

Price: $66

Product PDFs

Datasheet:

Iron is one of the essential trace elements in human body, which is the main component of hemoglobin,

myoglobin, cytochrome and other enzyme systems. Iron can assist in the transport of oxygen and promote

fat oxidation. Iron deficiency can easily cause anemia, metabolic disorders, and affect the immune function

of the body.

Fe³⁺is reduced by sodium sulfite to Fe²⁺, which reacts with 2,2-dipyridine-bipyridine, have an absorption

peak at 520 nm. According measure absorbance at 520 nm can reflect tissue iron concentration.

Reagents and Equipment Required but Not Provided.

Microplate reader or spectrophotometer, centrifuge, chloroform, adjusted transferpettor, micro glass

cuvette/96 well flat-bottom plate, mortar/homogenizer, chloroform, ice and distilled water.

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