DPPH free radical is a very stable nitrogen-centered free radical. It is one of the important indicators of the
sample's antioxidant capacity and is widely used in the research of antioxidant foods, health products and
pharmaceuticals.
The DPPH radical has a single electron, and its alcohol solution is purple, with strong absorption at 515 nm.
When an antioxidant present, DPPH free radicals are cleared, the solution color becomes lighter, and the
absorbance at 515 nm decreases. Within a certain range, the change in absorbance is directly proportional to
the degree of free radical removal. In this kit, the ability of the sample to remove DPPH free radicals is
reflected by the degree of decrease in absorbance.
Reagents and Equipment Required but Not Provided.
Spectrophotometer/ microplate reader, water bath, centrifuge, micro glass cuvette/ 96 well flat-bottom plate,
motor/ grinder, absolute ethanol, oven, 30-50 mesh sieve and distilled water.
