Catalase (CAT) Activity Assay Kit （Ammonium Molybdate-Chromogenic Method）

Home > Product > Test Kit > Catalase (CAT) Activity Assay Kit （Ammonium Molybdate-Chromogenic Method）

过氧化氢酶（CAT）活性检测试剂盒（钼酸铵法)

Cat:

NA0229

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/24S

Storage instructions：

2-8℃

Product Overview：

Components:

Extracting solution: Liquid 50 mL×1. Storage at 4℃.

Reagent I: Liquid 25 mL×1. Storage at 4℃.

Reagent Ⅱ: Powder×2. Storage at 4℃. Before use, take a bottle of Reagent Ⅱ and add 17.5 mL of distilled

water to dissolve it completely. Keep the unused reagent at 4℃ for one week.

Standard solution: Liquid 0.5 mL×1. Storage at 4℃. Preparation of 20 μmol/mL standard solution: the

standard solution provided by this kit is 1 mmol/mL H₂O₂standard solution. Before use, take 0.2 mL of

standard solution and add 9.8 mL of Reagent Ⅰ to dilute it or prepare it according to the proportion of

sample size, and mix it well. That is 20 μmol/mL standard solution, prepare when the solution will be used.

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Unit:

Price: $24

Product PDFs

Datasheet:

CAT (EC 1.11.1.6) widely exists in animals, plants, microorganisms and cultured cells. It is the most

important H₂O₂scavenging enzyme and plays an important role in the scavenging system of reactive

oxygen species.

H₂O₂can react with ammonium molybdate to form a stable yellow complex with a strong absorption

peak at 405 nm, and its absorption value is proportional to the concentration of hydrogen peroxide. By

measuring the amount of residual H₂O₂in the reaction system, the amount of H₂O₂catalyzed by CAT is

obtained, which reflected the activity of CAT.

Reagents and Equipment Required but Not Provided:

Spectrophotometer, desktop centrifuge, adjustable pipette, constant temperature water bath, 1 mL glass

cuvette, mortar/homogenizer, ice and distilled water.

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