Creatinine（Cr）Content Assay Kit

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肌酐（Cr）含量检测试剂盒（肌氨酸氧化酶法）

Cat:

NA0215

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extracting solution I：Liquid 60 mL×1. Storage at 4℃.

Extracting solution Ⅱ：Liquid 8 mL×1. Storage at 4℃.

Reagent I: Powder ×2. Storage at -20℃. Before use, add 1.7 mL of distilled water to each tube and

dissolve it completely. The unused reagents can be divided and then stored at -20℃ in two weeks.

Reagent Ⅱ: Powder ×2. Storage at -20℃. Before use, add 1.65 mL of distilled water to each tube and

dissolve it completely. The unused reagents can be divided and then stored at -20℃ in two weeks.

Reagent Ⅲ: Powder ×2. Storage at -20℃. Before use, add 1 mL of distilled water to each tube（50T/48S）

and dissolve it completely. For the convenience of storage, give one more. The unused reagents can

be divided and then stored at -20℃ in two weeks.

Reagent Ⅲ working solution: according to the required amount of the test, according to the ratio of

Reagent Ⅲ: distilled water = 1:9, fully mix and use now.

Reagent Ⅳ: Powder ×1. Storage at 4℃. Before use, add 1 mL of distilled water and dissolve it completely.

The unused reagents is divided and then stored at -20℃.

Reagent Ⅴ: Powder ×2. Storage at -20℃. Before use, add 3.4 mL of distilled water to each tube and

dissolve it completely. The unused reagents is divided and then stored at -20℃.

Reagent ⅥA: Liquid 10 mL ×1. Storage at 4℃.

Reagent ⅥB: Liquid 10 mL ×1. Storage at 4℃. Before use, according to the amount required by the

experiment, the mixture shall be fully mixed according to the ratio of Reagent ⅥA: Reagent ⅥB =

1:1, and prepare when the solution will be used.

Standard solution: Powder ×1, 1 mg of creatinine. Before use, add 1 mL of distilled water to fully dissolve,

i.e. 1 mg/mL standard solution. Before use, 50 μL of 1 mg/mL standard solution and 200 μL of

distilled water are mixed to prepare a standard solution of 200 μg/mL, prepare when the solution will

be used.

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Unit:

Price: $367

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
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Creatinine (Cre), the chemical formula is C₄H₇N₃O, is a product of muscle metabolism in the human body,

which is mainly excreted by glomerular filtration. Serum creatinine comes from both exogenous and

endogenous sources. Exogenous creatinine is the product of meat metabolism in the body; endogenous

creatinine is the product of muscle metabolism in the body.

The creatinine in the sample is hydrolyzed to creatine under the catalysis of creatinine enzyme. Under the

catalysis of creatinase, creatine hydrolyzes to creatine and urea. Sarcosine is oxidized to hydrogen peroxide

under the catalysis of sarcosine oxidase. Peroxidase catalyzed the oxidation of 4-aminoantipyrine coupling

phenol by hydrogen peroxide to form colored compounds with characteristic absorption peak at 505nm.

Reagents and Equipment Required but Not Provided:

Spectrophotometer, low tempareture centrifuge, transferpettor, 1 mL glass cuvette, mortar/homogenizer,

ice and distilled water, ultrasonic crusher.

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