Creatine is a nitrogen-containing compound, which is naturally found in vertebrates, and can assist in
energy supply for muscle and nerve cells. Creatine can be synthesized by three amino acids, arginine,
glycine and methionine, which can be synthesized by human body or taken from food. About 95% of
creatine is found in skeletal muscle, mainly in the form of phosphocreatine. As a supplement, creatine can
enhance the performance of the muscles by increasing the muscle quality. Creatine is also widely studied
as a therapeutic drug for neuromuscular diseases, which may help to protect the nerves and improve the
biological function of cells.
Creatine can react with diacetyl-α-naphthol in alkaline condition to form a red product with an absorption
peak at 530 nm.
Reagents and Equipment Required but Not Provided:
Spectrophotometer/Microplate reader, low tempareture centrifuge, transferpettor, micro glass cuvette/96
well plate, mortar/homogenizer, ice and distilled water.
