Creatine is a nitrogen-containing compound, which is naturally found in vertebrates, and can assist in
energy supply for muscle and nerve cells. Creatine can be synthesized by three amino acids, arginine,
glycine and methionine, which can be synthesized by human body or taken from food. About 95% of
creatine is found in skeletal muscle, mainly in the form of phosphocreatine. As a supplement, creatine can
enhance the performance of the muscles by increasing the muscle quality. Creatine is also widely studied
as a therapeutic drug for neuromuscular diseases, which may help to protect the nerves and improve the
biological function of cells.
Creatine can be converted into glycine, formaldehyde and hydrogen peroxide by creatine enzyme
coupled with sarcosine oxidase. Peroxidase catalyzes hydrogen peroxide to oxidize 4-aminoantipyrine
coupled phenol to form colored compounds with characteristic absorption peak at 505 nm.
Reagents and Equipment Required but Not Provided:
BC4935 -- page 1 / 4
