Formaldehyde dehydrogenase exists in most prokaryotes and all eukaryotes. It is an oxidoreductase that
converts formaldehyde. Formaldehyde dehydrogenase can catalyze formaldehyde and NAD+ to produce
NADH. The absorbance at 340 nm will increase. By measuring the change at 340nm, the activity of
formaldehyde dehydrogenase can be calculated.
Reagents and Equipment Required but Not Provided:
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, pipette, micro quartz cuvette/96 well UV
plate, mortar/homogenizer, ice and distilled water.