Wine is a general term for alcoholic (ethanol) beverages, and ethanol is the main component of wine
and one of the important indicators to measure the quality of wine. Ethanol can be used in the manufacture
of acetic acid, beverages, flavors, dyes, fuels, etc. Ethanol with a volume fraction of 70% to 75% is
commonly used as a disinfectant in medicine. Ethanol has a wide range of uses in the chemical industry,
medical and health, food industry, agricultural production and other fields.
Ethanol is oxidized under the catalysis of alcohol oxidase to produce hydrogen peroxide. Peroxidase
catalyzes the oxidation of hydrogen peroxide to 4-aminoantipyrine to couple phenol to generate a colored
compound with a characteristic absorption peak at 505nm. The change of the absorption peak at 505nm can
be measured to reflect the ethanol content.
Reagents and Equipment Required but Not Provided：
Spectrophotometer/microplate reader, desk centrifuge, constant temperature incubator/water bath,
pipette, micro glass cuvette/96 well plate, mortar/homogenizer, ice and distilled water.
