Direct bilirubin (DBIL) is also called conjugated bilirubin. After indirect bilirubin enters the liver, it is
combined with glucuronic acid by the action of glucuronosyltransferase in the liver. The increase of direct
bilirubin is of great significance for clinical diagnosis of obstructive jaundice, hepatocellular jaundice, liver
cancer, pancreatic head cancer, cholelithiasis and cholangiocarcinoma. Direct bilirubin could be oxidized
by sodium nitrite to form biliverdin, which has absorbance in 450 nm. The content of direct bilirubin can
be calculated by detecting the wavelength change at 450 nm.
Reagents and Equipment Required but Not Provided：
Spectrophotometer, desk centrifuge, constant temperature foster box/water-bath, pipette, 1mL glass
cuvette, ice and distilled water.
