Total bilirubin (TBIL) is the sum of direct bilirubin and indirect bilirubin. Determination of serum
total bilirubin is an important item in liver and gallbladder function tests. The content of direct bilirubin is
of great significance for clinical diagnosis of latent jaundice. Total bilirubin could be oxidized by sodium
nitrite to form biliverdin, which has absorbance in 450 nm. The content of total bilirubin can be calculated
by detecting the wavelength change at 450 nm.
Reagents and Equipment Required but Not Provided：
Spectrophotometer/Microplate reader, desk centrifuge, constant temperature foster box/water-bath,
pipette, micro glass cuvette/96 well flat-bottom plate, ice and distilled water.
