Polyamine Oxidase （PAO）Activity Assay Kit

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多胺氧化酶（PAO）活性检测试剂盒

Cat:

NA0166

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

2-8℃

Product Overview：

Components:

Extract solution: Liquid 110 mL×1. Store at 2-8℃.

Reagent I: Liquid 15 mL×1. Store at 2-8℃.

Reagent II: Liquid 1.3 mL×1. Store at 2-8℃.

Reagent III: Liquid 1.3 mL×1. Store at 2-8℃.

Reagent IV: Liquid 1.1 mL×1. Store at -20℃. It could be divided into small tubules and stored at -

20℃ after thawing to avoid repeated freezing and thawing.

Working solution: Reagent I, Reagent II and Reagent III are mixed by the ratio of 600μL:60μL:60μL

（about 5T）to make working solution according to sample number. Prepare when the solution will be

used.

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Unit:

Price: $359

Product PDFs

Datasheet:

Polyamine Oxidase (PAO) is a key enzyme which catalyse the aerobic degradation of polyamines.

Polyamines have strong binding capacity to nucleic acid, protein and cell membrane molecules and

directly affect cell growth, differentiation and apoptosis. PAO could be involved in the response to stress

and development of plant by regulating polyamines content.

PAO catalyzes polyamines to produce H₂O₂. Peroxidase catalyzes the production of H₂O₂into oxygen

and oxidizes o-dianisidine to form colored substance which has light absorption at 500nm. The color depth

is linear with PAO activity.

Reagents and Equipment Required but Not Provided:

Spectrophotometer/Microplate reader, balance, constant temperature foster box/water-bath,

centrifuge,

micro

glass

cuvette/96-well

flat-bottom

plate,

adjustable

transferpettor,

mortar/homogenizer/cell ultrasonic crusher, ice and distilled water.

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