Lipid Peroxide (LPO) Content Assay Kit

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脂质过氧化物（LPO）含量检测试剂盒

Cat:

NA0164

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

2-8℃

Product Overview：

Components:

Extract solution: Liquid 100 mL×1. Store at 2-8℃.

Reagent I: Liquid 11 mL×1. Store at 2-8℃.

Reagent II: Powder×2. Store at 2-8℃. Before use, take one and add 7 ml distilled water to fully

dissolve it. This reagent is difficult to dissolve. It can be heated at 70 ℃ and vibrated violently to promote

dissolution, or it can be treated by ultrasound to promote dissolution. Before each use, check whether there

is powder precipitation. The unused reagent can be stored at 2-8℃ for 1 month

Reagent III: Liquid 4 mL×1. Store at 2-8℃.

Standard Solution: Liquid 1 mL×1. Store at 2-8℃. Standard solution with concentration of

1000nmol/mL.

Diluent: Liquid 20 mL×1. Store at 2-8℃.

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Unit:

Price: $231

Product PDFs

Datasheet:

Lipid Hydroperoxide (LPO) is a kind of peroxide produced by unsaturated fatty acid chain through

the action of free radicals or reactive oxygen species. Under pathological conditions, the enhancement of

lipid peroxidation can lead to the increase of LPO with low content. The increase of LPO content will

damage the structure and function of cells. LPO content is closely related to the immune system and aging.

LPO is heated under acidic conditions to produce malondialdehyde (MDA). MDA is condensed with

thiobarbituric acid (TBA) to produce a brownish red substance named 3,5,5-trimethyloxazole-2,4-dione.

Its maximum absorption wavelength is 532nm. The content of LPO in the sample can be estimated after

colorimetry.

Reagents and Equipment Required but Not Provided:

Spectrophotometer/microplate reader, desk centrifuge, adjustable transferpettor, water bath, micro

quartz cuvette/96 well flat-bottom UV plate, mortar/homogenizer, ice, distilled water.

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