Plant Root Vitality （TTC-Method） Assay Kit

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植物根系活力检测试剂盒（TTC法）

Cat:

NA0160

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

2-8℃

Product Overview：

Components:

Reagent I A: powder×2. Storage at 2-8 ℃.

Reagent I B: powder×2. Storage at 2-8 ℃. Before use, take a bottle of reagent I B and add it to a bottle

of reagent I A, and add 30mL of reagent II to dissolve. The reagents were prepared before the experiment.

After preparation, the reagent should be stored at 2-8°C and used within a week. If the reagent turns red, it

cannot be used.

Reagent II: 125mL×1. Storage at 2-8 ℃.

Reagent III: powder×3. Storage at room temperature.

Reagent IV: 120 mL×1. Storage at 2-8 ℃. Ethyl acetate, self-provided reagents. Provide a 125 mL

brown bottle .

Standard: powder×1, storage at 2-8℃. Before use, add 1 mL of reagent II, shake and mix well, (10

mg/mL TTC standard solution). The reagent can be stored at 2-8°C for 1 week, if it appears red, it cannot be

used .

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Unit:

Price: $108

Product PDFs

Datasheet:

The root is the main organ for plants to absorb water and mineral nutrients, and it is also the organ for

the synthesis, assimilation and transformation of important substances in plants such as amino acids and

hormones. Therefore, the growth and activity of roots directly affect the growth of individual plants. Nutrient

level and yield level, etc. Root vigor has important practical significance.

TTC can be reduced to insoluble red triphenylformazan (TTF) by hydrogen, and TTF has an absorption

peak at 485 nm. When the TTC solution penetrates into the root tissue of the plant, the reducing substances

produced by the respiration process can reduce it to TTF (red), and the root tissue of the plant is stained red.

The root vigor can be expressed by the reduction amount of TTC. The root activity detected by this method

is the dehydrogenase activity of plant roots.

Reagents and Equipment Required but Not Provided:

Spectrophotometer/microplate reader, centrifuge, water bath/incubator, adjustable pipette, micro glass

cuvettes/96-well plates (non-polystyrene), mortar/homogenizer, ethyl acetate, ice and distilled water.

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