Glutamine (Gln) Content Assay Kit

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谷氨酰胺（Gln）含量检测试剂盒

Cat:

NA0148

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract Solution I: Liquid 80 mL×1. Storage at 2-8℃.

Extract Solution II: Liquid 30 mL×1. Storage at 2-8℃. Chloroform, self prepared.

Reagent I: Liquid 2 mL×1. Storage at 2-8℃.

Reagent II: Powder×1. Storage at -20℃. Before use, take one and add 0.2 mL distilled water to fully

dissolve it, the unused reagent can be stored at 4℃ for 4 weeks. Avoid repeated freezing and thawing.

Reagent III: Liquid 5 mL×1. Storage at 2-8℃.

Reagent Ⅳ: Powder×1. Storage at -20℃. Before use, add 20 mL Extract Solution I to fully dissolve

it. The unused reagent can be stored at 2-8℃ for 4 weeks. Avoid repeated freezing and thawing.

Reagent Ⅴ: Powder×1. Storage at -20℃. Before use, add 1.5 mL Reagent I to fully dissolve it. The

unused reagent can be stored at 2-8℃ for 4 weeks. Avoid repeated freezing and thawing.

Reagent ⅤI: Liquid 4 mL×1. Storage at 2-8℃.

Standard Solution: Liquid 1 mL×1. storage at 2-8℃. Glutamine standard solution with

concentration of 10 μmol/mL.

Reagent II Working Solution: An empty brown reagent bottle is provided in the kit. Dilute

according to the sample size according to the ratio of Reagent II: distilled water=0.05 mL: 0.7 mL (about

18 samples), The reagent should be prepared just before use, and put it on ice when using.

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Unit:

Price: $1175

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
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Glutamine is the amide of glutamic acid and one of the important amino acids that make up proteins.

At the same time, glutamine is also the main source of α-ketoglutaric acid in the tricarboxylic acid cycle.

Glutamine exists in the organism in two states: free state and bound state. Free glutamine plays an

important role in organism metabolism, and its metabolism accounts for more than 60% of free amino

acids in cells and blood circulation.

Free glutamine is converted to glutamic acid under the catalysis of glutaminase, Under the catalysis of

glutamate dehydrogenase (GDH), glutamate and NAD are produced α- Ketoglutaric acid, NADH and

NH4⁺, under the action of 1-mPMS, WST can react with NADH to produce water-soluble formazan, which

has the maximum absorption peak at 450 nm, and the glutamine content can be calculated.

Reagents and Equipment Required but Not Provided:

Microplate Reader/Spectrophotometer, Desktop Centrifuge, Water Bath/Constant Temperature

Incubator, Transferpettor, Mortar/Homogenizer/Cell Ultrasonic Crusher, Micro Glass Cuvette/96 Well

Flat-bottom Plate, Chloroform and Distilled Water.

BC5305 -- Page 1 / 4

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