Iron is one of the essential trace elements in human body, which is the main component of
hemoglobin, myoglobin, cytochrome and other enzyme systems. Iron can assist in the transport of oxygen
and promote fat oxidation. Iron deficiency can easily cause anemia, metabolic disorders, and affect
the immune function of the body.
Fe3+ is reduced by hydroxylamine hydrochloride to Fe2+. Fe2+ could react with Phenanthroline
Monohydrate to form a kind of orange compound under weak acid condition, which has an absorption
peak at 510 nm. According measure absorbance at 520 nm can reflect cell iron concentration.
Reagents and Equipment Required but Not Provided:
Spectrophotometer/Microplate reader, desk centrifuge, adjustable pipette, micro glass cuvette/96 well
flat-bottom plate, cell ultrasonic crusher, ice, distilled water.