High-Density Lipoprotein Cholesterol（HDL-C）Content Assay Kit

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高密度脂蛋白胆固醇（HDL-C）含量检测试剂盒

Cat:

NA0152

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

2-8℃

Product Overview：

Components:

Extract I: Isopropyl alcohol 110 mL ×1. Required but not provided. Store at 2-8℃.

Extract ⅡA: Liquid 6 mL×1. Store at 2-8℃.

Extract ⅡB: Liquid 6 mL×1. Store at 2-8℃. According to the required amount of the experiment,

according to the ratio of extract ⅡA: extract ⅡB =50 μL: 50 μL (1T amount), mix well, and prepare before

use.

Reagent IA: Powder ×2. Store at 2-8℃.

Reagent IB: Liquid 30 mL×1. Store at 2-8℃. Add 14mL of Reagent IB to one Reagent IA and shake

to dissolve. It could be stored at 2-8℃ for two weeks.

Reagent Ⅱ: Liquid 160 μL×1. Store at 2-8℃.

Reagent Ⅲ: Liquid 25 μL×1. Store at 2-8℃.

Standard Solution: Powder ×1, 10 mg cholesterol. Store at 2-8℃. Add 517 μL of Extract I before

use and shake to dissolve. The cholesterol standard solution of 50 μmol/mL could be stored at 2-8℃ for

four weeks.

Working Solution: According to the required amount of the experiment, according to the ratio of

Reagent I: Reagent Ⅱ: Reagent Ⅲ=3mL: 20 μL: 3 μL (16T amount), mix well, and prepare before use

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Unit:

Price: $132

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
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High-density lipoprotein (HDL) is a lipoprotein that contains a small amount of cholesterol and

carries cholesterol away from body cells and tissues. High-density lipoprotein cholesterol (HDL-C)

concentrations negatively correlate with the incidence of atherosclerosis and coronary heart disease.

Therefore, accurate and precise measurements of patients’ HDL-C concentrations are necessary to

appropriately identify individuals with atherosclerosis, coronary heart disease and hypertension.

Cholesterol is specifically dissociated by one surfactant from HDL. Esterase can catalyze the

hydrolysis of cholesterol ester to produce free cholesterol (FC) and free fatty acid (FFA), thus transforming

cholesterol ester into FC; Furthermore, cholesterol oxidase can catalyze FC to form Δ4-cholesterone and

H₂O₂; Finally, peroxidase can catalyze the oxidation of 4-aminoantipyrine and phenyl amines by H₂O₂to

form purple quinones. It has a characteristic absorption peak at 546 nm, and its color depth is directly

proportional to cholesterol content.

Reagents and Equipment Required but Not Provided.

Spectrophotometer/microplate reader, balance, low temperature table centrifuge, constant temperature

incubator/water bath, micro glass cuvette/96 well flat-bottom plate, pipette, mortar/homogenizer/cell

BC5325 – Page 1 / 3

ultrasonic crusher, ice, distilled water, isopropyl alcohol.

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