Sialic Acid Content Assay Kit

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土壤植酸酶活性检测试剂盒

Cat:

NA0135

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/24S

Storage instructions：

2-8℃

Product Overview：

Components:

Reagent I: Liquid 2.5 mL×1, storage at 2-8℃ . Toluene (self-provided).

Reagent II A: Powder×1, storage at 2-8℃.

Reagent II B: Liquid 50 mL×1, storage at 2-8℃. Pour all reagent II B into reagent II A before use, fully

shake and dissolve it, the unused reagent can be stored at 2-8℃ for 4 weeks.

Reagent III: Powder×1, storage at 2-8℃. Before use, add 7 mL distilled water to fully dissolve it. Then

slowly add 1.89mL concentrated sulfuric acid under the liquid level. The unused reagent can be stored at

2-8℃ for 4 months.

Reagent IV: Powder×1, storage at 2-8℃. Before use, add 35 mL distilled water to fully dissolve it.

Then slowly add 50μL concentrated sulfuric acid. The unused reagent can be stored at 2-8℃ for 4 months.

Working Solution: Before use, mix the proportion of Reagent III: Reagent IV=1mL: 5mL (about 12T,

which can be adjusted proportionally according to the sample size), the unused reagent can be stored at 2-

8℃ for 3 days.

Standard: Liquid 2 mL×1, storage at 2-8℃. 5 μmol/mL inorganic phosphorus standard solution.

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Unit:

Price: $141

Product PDFs

Datasheet:

Phytase, also known as inositol hexaphosphatase, is an enzyme that combines protein and sugar. Phytase

can decompose phytic acid to produce inorganic phosphorus and inositol, greatly improving the utilization

of nutrients by organisms. Soil phytase mainly comes from soil microorganisms and plays an important

role in the phosphorus cycle. Soil phytase has a strong application prospect in the field of soil improvement

and sustainable agricultural development.

Under certain environmental conditions, soil phytase can decompose sodium phytate (twelve sodium

inositol hexaphosphate) to produce inorganic phosphorus and inositol derivatives. Under acidic conditions,

inorganic phosphorus reacts with ammonium molybdate chromogenic agent to produce blue molybdenum

blue substance, which has a characteristic absorption peak at 700nm. The activity of soil phytase can be

calculated by measuring the content of inorganic phosphorus.

Reagents and Equipments Required but Not Provided:

Spectrophotometer, centrifuge, water-bath/constant temperature incubator, transferpettor, 1 mL glass

cuvette, oscillator, toluene, concentrated sulfuric acid, mortar, 30-50 mesh sieve , ice and distilled water.

BC5370 -- Page 1 / 4

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