Pyrroline-5-Carboxylate Reductase（P5CR）Activity Assay Kit

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吡咯啉-5-羧酸还原酶（P5CR）活性检测试剂盒

Cat:

NA0144

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution I:60 mL×1. Storage at 2-8℃.

Extract solution II:600 μL×1. Storage at -20℃. Reagents are volatile, seal as soon as possible after

use and put back to -20℃ for storage. The preparation of the extraction solution: according to the sample

volume the extraction solution I extraction solution II = 0.01mL: 0.99mL (1T) ratio of the preparation of

the extraction solution, ready to use, forbidden to prepare in advance.

Reagent I: 5 mL×1. Storage at 2-8℃.

Reagent II: 10mL×1. Storage at 2-8℃.

Reagent III: powder×1. Storage at -20℃. Add 5.6mL of reagent I before use and mix thoroughly.

The unused reagents can be stored in separate packages at -20℃ for 4 weeks, avoid repeated freezing and

thawing.

Reagent IV:4mL×1. Storage at 2-8℃.

Standard: powder×1, storage at -20℃. Add 1.4 mL distilled water before use0. 2 µmol/mL NADH

standard solution. The unused reagents should be stored in separate containers at -20℃ for 2 weeks,

avoiding repeated freezing and thawing during the period. Before use, take 100 μL of 2 µmol/mL NADH

standard solution in EP tube, add 700 μL of distilled water to dissolve fully, and prepare 0.25 μmol/mL

NADH standard solution for use.

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Unit:

Price: $396

Product PDFs

Datasheet:

Pyrroline-5-carboxylate reductase (P5CR) is an important housekeeping protein widely found in

prokaryotes and eukaryotes. In the presence of NAD(P)H, pyrroline-5-carboxylic acid (P5C) is converted

to proline by pyrroline-5-carboxylate reductase, and P5CR has also been found to be involved in the

metabolism of thioproline in E. coli.

Thioproline is dehydrogenated by pyrroline-5-carboxylate reductase and is accompanied by the

conversion of NAD to NADH. In the presence of 1-mPMS, WST-1 reacts with NADH to produce water-

soluble formazan with a characteristic absorption peak at 450 nm.

Reagents and Equipment Required but Not Provided:

Spectrophotometer, water bath/constant incubator, adjustable pipette, analytical balance,

mortar/homogenizer/cell sonicator , micro glass cuvettes/96 well plates, distilled water and ice.

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