Home > Product > Test Kit > Carbonic Anhydrase Activity Assay Kit
碳酸酐酶活性检测试剂盒
Cat:
NA0127
Assay Type:
Spectrophotometer
Brand:
sunlong medical
Specificity:
50T/48S
Storage instructions:
2-8℃
Product Overview:
Components:  
Extract solution:60 mL×1. Storage at 2-8℃.  
Reagent I: 50 mL×1. Storage at 2-8℃.  
Reagent II: powder×2. Storage at -20℃; Before use, take a bottle of Reagent II, add 320μL acetone  
to shake and dissolve, the reagent can be divided and stored for 1 week in -20℃, avoid repeated freeze-  
thaw;  
Reagent II working solution: before use according to the ratio of reagent II: distilled water = 40μL:  
960μL (5T) to mix and prepare into reagent II working solution.  
Standard: 1 mL×1. Storage at 2-8℃. 5 μmol/mL phenol standard solution. Before use, take 100 μL  
of 5 μmol/mL phenol standard solution in an EP tube, add 1500 μL of distilled water and mix thoroughly  
to make 0.3125 μmol/mL of phenol standard solution.  
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Unit:
Price: $
Product PDFs
Datasheet:


Carbonic anhydrase (CA, EC4.2.1.1) is a metalloenzyme with Zn2+ as the active center, which can  
-
efficiently catalyze the reversible hydration reaction of CO2: CO2+H2O HCO +H+ at a rate 107 times  
3
higher than that under natural conditions, which is one of the fastest known enzymes. It is one of the fastest  
known enzymes.  
Carbonic anhydrase can catalyze the reaction of p-nitrophenyl acetate to form p-nitrophenol, and the  
rate of increase of absorbance value at 405 nm reflects the activity of carbonic anhydrase.  
Reagents and Equipment Required but Not Provided:  
Spectrophotometer, centrifuge, water bath/incubator, adjustable pipette, 1mL glass cuvette,  
mortar/homogenizer/cell sonicator, ice and distilled water.  
 
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