Carbonic Anhydrase Activity Assay Kit

Home > Product > Test Kit > Carbonic Anhydrase Activity Assay Kit

碳酸酐酶活性检测试剂盒

Cat:

NA0126

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

2-8℃

Product Overview：

Components:

Extract solution:120 mL×1. Storage at 2-8℃.

Reagent I: 20 mL×1. Storage at 2-8℃.

Reagent II: powder×2. Storage at -20℃; Before use, take a bottle of Reagent II, add 200μL acetone

to shake and dissolve, the reagent can be divided and stored for 1 week in -20℃, avoid repeated freeze-

thaw;

Reagent II working solution: before use according to the ratio of reagent II: distilled water = 20μL:

480μL (12T) to mix and prepare into reagent II working solution. Reagents are prepared at the time of use.

Standard: 1 mL×1. Storage at 2-8℃. 5 μmol/mL phenol standard solution. Before use, take 100 μL

of 5 μmol/mL phenol standard solution in an EP tube, add 700 μL of distilled water and mix thoroughly to

make 0.625 μmol/mL of phenol standard solution.

prev next

Unit:

Price: $257

Product PDFs

Datasheet:

Carbonic anhydrase (CA, EC4.2.1.1) is a metalloenzyme with Zn²⁺as the active center, which can

efficiently catalyze the reversible hydration reaction of CO²: CO₂+H₂O ⇋HCO +H⁺at a rate 10⁷times

higher than that under natural conditions, which is one of the fastest known enzymes. It is one of the fastest

known enzymes.

Carbonic anhydrase can catalyze the reaction of p-nitrophenyl acetate to form p-nitrophenol, and the

rate of increase of absorbance value at 405 nm reflects the activity of carbonic anhydrase.

Reagents and Equipment Required but Not Provided:

Spectrophotometer/microplate reader, centrifuge, water bath/incubator, adjustable pipette, Micro glass

cuvettes/96 well plates , mortar/homogenizer/cell sonicator, ice and distilled water..

Product Feedback Wall