Fluoride Resistant Acid Phosphatase Activity Assay Kit

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抗氟离子酸性磷酸酶（FRAP）活性检测试剂盒

Cat:

NA0132

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution:60 mL×1. Storage at 2-8℃.

Reagent I: 1.5 mL×1. Storage at 2-8℃.

Reagent II: 1.2 mL×1. Storage at 2-8℃.

Reagent III: powder×2,. Storage at -20℃. Before use, add 1mL of water, dissolve it fully. Unused

reagents can be stored at -20℃ for 4 weeks.Avoid repeated freezing and thawing. One reagent can do

100T after dissolving. To prolong the use of the kit, therefore, one more powder is given.

Reagent IV: powder×1. Storage at 2-8℃. Before use, add 5.5mL of water, dissolve it fully. Unused

reagents can be stored at 2-8℃ for 4 weeks.

Reagent V : 0.3 mL×1. Storage at 2-8℃. According to the ratio of reagent V: distilled water = 1:9

according to the number of samples before use.

Reagent VI: 1.2 mL×1. Storage at 2-8℃.

Reagent VII: 1.2 mL×1. Storage at 2-8℃.

Reagent VIII: 15 mL×1. Storage at 2-8℃.

Standard: 1 mL×1. Storage at 2-8℃. 5 μmol/mL phenol standard solution. Before use, take 100 μL

of 5 μmol/mL phenol standard solution in an EP tube, add 300 μL of distilled water and mix thoroughly to

make 1.25 μmol/mL phenol standard solution..

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Unit:

Price: $86

Product PDFs

Datasheet:

Fluoride resistant acid phosphatase (FRAP) is a type of acid phosphatase. FRAP is mainly found in

the lysosomes of most cells, the prostate gland, brain, liver, spleen, and platelets.

The activity of fluoride-resistant acid phosphatase is not inhibited by fluoride ions, whereas the

activity of other acid phosphatases is inhibited by fluoride ions. Under acidic conditions, the fluoride-

resistant ion acid phosphatase catalyzes the production of p-nitrophenol from PNPP. The p-nitrophenol

appears yellow under alkaline conditions and can be detected at 400 nm absorbance. The darker the yellow

color of the product, the higher the activity of the fluorine-resistant ion acid phosphatase, and vice versa,

the lower the enzyme activity.

Reagents and Equipment Required but Not Provided:

Spectrophotometer/microplate reader, centrifuge, water bath/incubator, adjustable pipette, Micro glass

cuvettes/96 well plates, mortar/homogenizer/cell sonicator, ice and distilled water

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