Mouse Anti-Beta tubulin (Loading Control) antibody

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Beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; Beta1 tubulin; Class I beta tubulin; M40; MGC117247; MGC16435; OK/SW cl.56; OK/SWcl.56; TBB5_HUMAN; TUBB 1; TUBB 2; TUBB 5; TUBB; TUBB1; TUBB2; TUBB5; tubulin beta 1 chain; Tubulin beta 2

Cat:

SLM33034M

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,Monkey,Insect, Yeast)

Immunogen：

KLH conjugated synthetic peptide derived from human Beta tubulin

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG1

Applications：

WB=1:5000-20000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100IF=1:200-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: U87MG Cell (Human) Lysate at 40 ugSW96 Cell (Human) Lysate at 40 ugPrimary: Anti- Beta tubulin (SLM33034M) at 1/2 000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDSample: Hela (Human) Lysate at 40 ugPrimary: Anti-Beta tubulin (SLM33034M) at 1/2000~1/20000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDSample: Cerebrum (Rat) Lysate at 40 ugPrimary: Anti-Beta tubulin (SLM33034M) at 1/1000~1/20000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDSample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-Beta tubulin (SLM33034M) at 1/1000~20000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDSample: Hela(Human) Cell Lysate at 30 ugNIH/3T3(Mouse) Cell Lysate at 30 ugHL60(Human) Cell Lysate at 30 ugSH-SY5Y(Human) Cell Lysate at 30 ugCerebellum(Mouse) Lysate at 40 ugCerebellum(Rat) Lysate at 40 ugCerebrum(Mouse) Lysate at 40 ugCerebrum(Rat) Lysate at 40 ugPrimary: Anti-Beta tubulin (SLM33034M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kDSample: HL60(Human) Cell Lysate at 30 ugPrimary: Anti- Beta tubulin (SLM33034M) at 1/2000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 52 kDSample: A431(Human) Cell Lysate at 30 ugSY5Y(Human) Cell Lysate at 30 ugPrimary: Anti- Beta tubulin (SLM33034M) at 1/2000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 52 kDSample: HL60(Human) Cell Lysate at 30 ugU937(Human) Cell Lysate at 30 ugA431(Human) Cell Lysate at 30 ugSH-SY5Y(Human) Cell Lysate at 30 ugPrimary: Anti- Beta tubulin (SLM33034M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kDSample: A431(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugJurkat(Human) Cell Lysate at 30 ugLovo(Human) Cell Lysate at 30 ugPrimary: Anti- Beta tubulin (SLM33034M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 52 kDParaformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (cell supernatant of SLM33034M 7G3) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (cell supernatant of SLM33034M 7G3) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (cell supernatant of SLM33034M 7G3) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (SLM33034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (SLM33034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (SLM33034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (ascites of SLM33034M 7G3) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (ascites of SLM33034M 7G3) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin) Monoclonal Antibody, Unconjugated (SLM33034M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (Beta tubulin) monoclonal Antibody, Unconjugated (SLM33034M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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Unit:

Price: $136.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]

Function:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit:
Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO. Interacts with MX1.

Subcellular Location:
Cytoplasmic, cytoskeleton.

Tissue Specificity:
Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Post-translational modifications:
Some glutamate residues at the SLCterminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).

Similarity:
Belongs to the tubulin family.

SWISS:
P07437

Gene ID:
203068

Database links:

Entrez Gene: 203068 Human

Entrez Gene: 22154 Mouse

Entrez Gene: 29214 Rat

Omim: 191130 Human

SwissProt: P07437 Human

SwissProt: P99024 Mouse

SwissProt: P69897 Rat

Unigene: 63696 Human

Unigene: 2458 Rat

Picture

Sample:
Hela (Human) Lysate at 40 ug
Primary:
Anti-Beta tubulin (SLM33034M) at 1/2000~1/20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary:
Anti-Beta tubulin (SLM33034M) at 1/1000~1/20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary:
Anti-Beta tubulin (SLM33034M) at 1/1000~20000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Sample:
Hela(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
HL60(Human) Cell Lysate at 30 ug
SH-SY5Y(Human) Cell Lysate at 30 ug
Cerebellum(Mouse) Lysate at 40 ug
Cerebellum(Rat) Lysate at 40 ug
Cerebrum(Mouse) Lysate at 40 ug
Cerebrum(Rat) Lysate at 40 ug
Primary: Anti-Beta tubulin (SLM33034M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Sample:
HL60(Human) Cell Lysate at 30 ug
Primary: Anti- Beta tubulin (SLM33034M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 52 kD

Sample:
A431(Human) Cell Lysate at 30 ug
SY5Y(Human) Cell Lysate at 30 ug
Primary: Anti- Beta tubulin (SLM33034M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 52 kD

Sample:
HL60(Human) Cell Lysate at 30 ug
U937(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
SH-SY5Y(Human) Cell Lysate at 30 ug
Primary: Anti- Beta tubulin (SLM33034M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

Sample:
A431(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Lovo(Human) Cell Lysate at 30 ug
Primary: Anti- Beta tubulin (SLM33034M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 52 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (cell supernatant of SLM33034M 7G3) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (cell supernatant of SLM33034M 7G3) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (cell supernatant of SLM33034M 7G3) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (SLM33034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (SLM33034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (SLM33034M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (ascites of SLM33034M 7G3) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Monoclonal Antibody, Unconjugated (ascites of SLM33034M 7G3) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin) Monoclonal Antibody, Unconjugated (SLM33034M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (Beta tubulin) monoclonal Antibody, Unconjugated (SLM33034M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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