Goat Anti-HBsAg antibody | Sunlong Medical

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L glycoprotein; L-HBsAg; LHB; Large S protein; Large surface protein; Major surface antigen; S; HBSAG_HBVC5.

Cat:

SL1557G

Species Reactivity：

Human,(predicted: Bee,Hepatitis B virus)

Immunogen：

Recombinant HBsAg protein

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Goat

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Hepatitis B virus infected human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HBsAg) Polyclonal Antibody, Unconjugated (SL1557G) at 1:200 overnight at 4°C, followed by A594 conjugated Rabbit Anti-Goat IgG H&L secondary antibody (SL0294R-A594) incubation and staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.

Function:
The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane (Probable). In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.
The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.

Subunit:
Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion.

Subcellular Location:
Virion membrane.

Post-translational modifications:
Isoform M is N-terminaly acetylated at a ratio of 90%, and N-glycosylated at the pre-S2 region.
Myristoylated.

Similarity:
Belongs to the orthohepadnavirus major surface antigen family.

SWISS:
P03140

Gene ID:
944569

Database links:

Entrez Gene: 2828293 Hepatitis B virus

Entrez Gene: 944569 Hepatitis B virus

SwissProt: P03138 Hepatitis B virus

SwissProt: P03140 Hepatitis B virus

SwissProt: P03141 Hepatitis B virus

SwissProt: Q81157 Hepatitis B virus

SwissProt: Q81158 Hepatitis B virus

SwissProt: Q81159 Hepatitis B virus

SwissProt: Q81160 Hepatitis B virus

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Specific References (13) | SL1557G has been referenced in 13 publications.

[IF=2.999] Okumura et al. Negative regulation of hepatitis B virus replication by forkhead box protein A in human hepatoma cells. (2015) FEBS.Lett. 589:1112-8 WB ; Human.

PubMed:25836733

[IF=4.368] Kong et al. The enhanced expression of death receptor 5 (DR5) mediated by HBV X protein through NF-kappaB pathway is associated with cell apoptosis induced by (TNF-α related apoptosis inducing ligand) TRAIL in hepatoma cells. (2015) Virol.J. 12:192 RT-PCR ; Human, Bee, Hepatitis B virus.

PubMed:26577955

[IF=5.23] Sakurai, Fuminori, et al. "Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection." Scientific Reports 7 (2017): 45698. IF(ICC) ; Human.

PubMed:28374759

[IF=4.18] Ito, H., et al. "Induction of humoral and cellular immune response to hepatitis B virus (HBV) vaccine can be upregulated by CpG oligonucleotides complexed with Dectin‐1 ligand." Journal of Viral Hepatitis (2016). IHSLCP ; Mouse.

PubMed:27807909

[IF=3.41] Yu, Hao-Tong, et al. "Comparison of the patterns of antibody recall responses to HISLV1 gp120 and hepatitis B surface antigen in immunized mice." Vaccine (2016). WB ; Mouse.

PubMed:27843002

[IF=5.02] Lai, M. W., et al. "Hepatocarcinogenesis in transgenic mice carrying hepatitis B virus pre-S/S gene with the sW172* mutation." Oncogenesis 5.12 (2016): e273. WB ; Mouse.

PubMed:27918551

[IF=5.23] Nakabori, T., et al. "Sodium taurocholate cotransporting polypeptide inhibition efficiently blocks hepatitis B virus spread in mice with a humanized liver."Scientific reports 6 (2015): 27782-27782. IHSLCF ; Human.

PubMed:27278060

[IF=2.03] Ma, Yan-Xiu, et al. "Successful establishment and evaluation of a new animal model for studying the hepatitis B virus YVDD mutant." Archives of virology (2012): 1-7. IHSLCP ; Mouse.

PubMed:23183771

[IF=14.079] Hayashi S et al. A novel association between core mutations in hepatitis B virus genotype F1b and hepatocellular carcinoma in Alaskan Native People. Hepatology.2018. CLEIA&ICF ; Human.

PubMed:10.1002/hep.30111

[IF=0] Gong et al. Construction and expression of a recombinant eukaryotic expression plasmid containing the preS1-preS2-S genes of hepatitis B virus and the granulocyte-macrophage colony stimulating factor gene: A study of its immunomodulatory effects. (2013) Biomed.Re. 1:251-256 WB ; Human.

PubMed:24648930

[IF=2.54] Xiang, A., et al. "A simple and rapid capillary chemiluminescence immunoassay for quantitatively detecting human serum HBsAg." European journal of clinical microbiology & infectious diseases 32.12 (2013): 1557-1564. ELISA ; Human.

PubMed:23780696

[IF=3.34] Okumura, Nobuaki, et al. "Negative regulation of hepatitis B virus replication by forkhead box protein A in human hepatoma cells." FEBS Letters (2015). WB ; Human.

PubMed:25836733

[IF=4.45] Watanabe, Tsunamasa, et al. "Post-Exposure Prophylactic Effect of HBSLVactive Antiretroviral Therapy Against Hepatitis B Virus Infection." Antimicrobial Agents and Chemotherapy (2014): AASLC04459. IF ; Human.

PubMed:25512419