Mouse Anti-Human Serum albumin (Loading Control) antibody

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ALB; ALBU_HUMAN; Albumin (32 AA); Albumin (AA 34); Analbuminemia; Bisalbuminemia; Cell growth inhibiting protein 42; DKFZp779N1935; Dysalbuminemic hyperthyroxinemia; Growth inhibiting protein 20; HSA; Hyperthyroxinemia dysalbuminemic; PRO0883; PRO0903; PR

Cat:

SLM0945M

Species Reactivity：

Human,Mouse,(predicted: Rat,Pig,)

Immunogen：

human Serum albumin

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG1

Applications：

WB=1:500-2000ELISA=1:1000-15000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample:Lung(Mouse)Lysate at 40 ugSpleen(Mouse)Lysate at 40 ugPrimary: Anti-Serum albumin(SLM0945M)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 69kDObserved band size: 69kDSample: Lane 1: Serum (Human) at 20 ugPrimary: Anti-Serum albumin (SLM0945M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 67 kDObserved band size: 67 kDSample:A549 (Human)Cell Lysate at 40 ugPrimary: Anti-Serum albumin(SLM0945M)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 69kDObserved band size: 69kDTissue/cell: human hepatocellular carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-ALB Monoclonal Antibody, Unconjugated(SLM0945M) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(SLC0010) staining

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Unit:

Price: $136.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Function:
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

Subcellular Location:
Secreted.

Tissue Specificity:
Plasma.

Post-translational modifications:
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid.

DISEASE:
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:10720]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.

Similarity:
Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains.

SWISS:
P02769

Gene ID:
280717

Database links:

Entrez Gene: 213 Human

Entrez Gene: 11657 Mouse

Entrez Gene: 24186 Rat

Omim: 10720 Human

SwissProt: P02768 Human

SwissProt: P07724 Mouse

SwissProt: P02770 Rat

Picture

Sample:
Lane 1: Serum (Human) at 20 ug
Primary: Anti-Serum albumin (SLM0945M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 67 kD
Observed band size: 67 kD

Sample:A549 (Human)Cell Lysate at 40 ug
Primary: Anti-Serum albumin(SLM0945M)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 69kD
Observed band size: 69kD

Tissue/cell: human hepatocellular carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ALB Monoclonal Antibody, Unconjugated(SLM0945M) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(SLC0010) staining

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