C; core; E; PC; pre-C; pre-C/C; PreC; PreC/C; precore-core; Capsid protein; Core and e antigen; Core antigen; Core protein; HBc; HBVgp4; Hepatitis B Virus core antigen; p21.5; precore/core protein; Q89714_HBV.
Cat:
SLM2000M
Immunogen：
Recombinded human hepatitis B core antigen protein
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Monoclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Mouse
Product Overview：
Sample: Lane 1: Recombinant HBcAg protein, Tag free Primary: Anti-HBcAg (SLM2000M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 21 kDaObserved band size: 25 kDa
Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 36 or 48 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection.
The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.
Function: Self assembles to form an icosahedral capsid. Mostcapsid appear to be large particles with a icosahedral symmetry ofT=4 and consist of 48 copies of capsid protein, though a fractionforms smaller T=3 particles consisting of 36 capsid proteins.Entering capsid are transported along microtubules to the nucleus.Phosphorylation of the capsid is thought to induce exposure ofnuclear localization signal in the SLCterminal portion of the capsidprotein that allows binding to the nuclear pore complex via theimportin (karyopherin-) alpha and beta. Capsids are imported inintact form through the nuclear pore into the nuclear basket, whereit probably binds NUP153. Only capsids that contain the matureviral genome can release the viral DNA and capsid protein into thenucleoplasm. Immature capsids get stucked in the basket. Capsidsencapsulate the pre-genomic RNA and the P protein. Pre-genomic RNAis reverse transcribed into DNA while the capsid is still in thecytoplasm. The capsid can then either be directed to the nucleus,providing more genome for transcription, or bud through theendoplasmic reticulum to provide new virions (By similarity). Encapsidates hepatitis delta genome.
Subunit: Homodimerizes, then multimerizes. Interacts with cytosolexposed regions of viral L glycoprotein present in thereticulum-to-Golgi compartment. Interacts with human FLNB.
Post-translational modifications: Phosphorylated by host SRPK1, SRPK2, and maybe protein kinaseC or GAPDH. Phosphorylation is critical for pregenomic RNApackaging. Protein kinase C phosphorylation is stimulated by HBxprotein and may play a role in transport of the viral genome to thenucleus at the late step during viral replication cycle.
Similarity: Belongs to the orthohepadnavirus core antigen family.
SWISS: Q89714
Gene ID: N/A
Database links:
乙肝核心抗原抗体（乙肝病毒核心抗原）用于HBV感染的肝组织。
Picture
Sample:
Lane 1: Recombinant HBcAg protein, Tag free
Primary: Anti-HBcAg (SLM2000M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 25 kDa
