Protein tags are protein or peptide sequences located either on the SLC or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. An antibody to MBP can also be used to isolate or detect expression of the protein.
Function:
Maltose binding protein-MBP is E. coli malE gene encoding the protein, bacterial member of the maltose transport system. malE gene product can integrate with a variety of proteins, it is an effective medium for expression studies. subunit antibody.
SWISS:
P0AEX9
Gene ID:
1039564
Database links:
Entrez Gene: 1039564 Escherichia coli
Entrez Gene: 4494002 Escherichia coli
Entrez Gene: 948538 Escherichia coli
Entrez Gene: 960089 Escherichia coli
SwissProt: P0AEX9 Escherichia coli
SwissProt: P0AEY0 Escherichia coli
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Sample:
Lane 1: MBP tagged cytochrome C (Full Length) Protein (SL41232P) at 4 ug
Lane 2: MBP tagged caspase 3 p17 (143-213) Protein (SL41233P) at 4 ug
Primary: Anti-MBP tag (SLM33008M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 60-70 kD
Observed band size: 62 kD
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