Sample:
Lane 1: EYFP-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: SL33182P) at 4 ug
Primary: Anti- EYFP-Tag (SLM33182M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD