Sample:
Lane1: HA-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2ug
Lane2: HA-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2ug
Primary: Anti-HA-Tag (SLM33003M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD