Sample:
Lane 1: mOrange-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: SL33183P) at 4 ug
Primary: Anti-mOrange-Tag (SLM33183M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD