Sample: Lane1: HA-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2ug Lane2: HA-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2ug Primary: Anti-HA-Tag (SLM33157M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD
Sample:
HA-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 4 ug
Primary: Anti-HA-Tag (SLM33157M) at 1/1000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Sample:
HA-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2 ug
Primary: Anti-HA-Tag (SLM33157M) at 1/1000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
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