Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five SLCterminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Function:
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Post-translational modifications:
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphrylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
Similarity:
Belongs to the ribosomal protein S6e family.
SWISS:
P62753
Gene ID:
6194
Database links:
Entrez Gene: 6194 Human
Entrez Gene: 20104 Mouse
Entrez Gene: 667739 Mouse
Entrez Gene: 29304 Rat
Omim: 3692 Human
SwissProt: P62753 Human
SwissProt: P62754 Mouse
SwissProt: P62755 Rat
Unigene: 408073 Human
Unigene: 379007 Mouse
Unigene: 425090 Mouse
Unigene: 34341 Rat
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Sample:
Lane 1: 293 cell lysates
Lane 2: Hela cell lysates
Lane 3: NIH/3T3 cell lysates
Lane 4: PSLC12 cell lysates
Lane 5: Rat brain tissue lysates
Primary: Anti-RPS6 (SLM51406M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size:29 kD
Observed band size:30 kD
Paraformaldehyde-fixed, paraffin embedded (Human brain section); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RPS6) Monoclonal Antibody, Unconjugated (SLM51406M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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