Mouse Anti-Flag Tag antibody

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DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; DYKDDDDK-Tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG-tag.

Cat:

SLM33346M

Species Reactivity：

Recombinant protein

Immunogen：

KLH conjugated Flag Tag

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:5000-20000ELISA=1:5000-10000IP=1:20-100IF=1:100-500not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample:DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 4 ugPrimary: Anti-Flag Tag (SLM33346M) at 1/1000 ~ 1/10000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 41 kDObserved band size: 41 kDSample:DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2 ugPrimary: Anti-Flag Tag (SLM33346M) at 1/1000 ~ 1/10000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 41 kDObserved band size: 41 kDSample: Lane1: DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate at 2ugPrimary: Anti-FLAG Tag (SLM33346M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 130 kDObserved band size: 130 kDSample: Lane1: DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate at 2ugLane2: DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate at 2ugPrimary: Anti-Flag Tag (SLM33346M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 41 kDObserved band size: 45 kD

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Unit:

Price: $136.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This is a useful tool for the localisation and characterisation of DDDDK tagged proteins.

SWISS:
N/A

Gene ID:
N/A

Picture

Sample:
DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2 ug
Primary: Anti-Flag Tag (SLM33346M) at 1/1000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD

Sample:
Lane1: DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Primary: Anti-FLAG Tag (SLM33346M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD

Sample:
Lane1: DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Lane2: DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Primary: Anti-Flag Tag (SLM33346M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD

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