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Home > Product > Antibody > Mouse Anti-human CD19 antibody
CD19_HUMAN; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen: CD19; B-lymphocyte surface antigen B4; Leu 12; Leu12;
Cat:
SLM30096M
Species Reactivity：
(predicted: Human,)
Immunogen：
KLH conjugated synthetic peptide derived from human CD19
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Monoclonal
Isotype：
Mouse IgG1
Applications：
Flow-Cyt=5ul/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Mouse
Product Overview：
scatter diagram showing peripheral blood lymphocytes stained with SLM30096M. The cells were incubated with the antibody (SLM30096M, 5 µl/Test) for 30 min at 22°C. The secondary antibody used for 40 min at room temperature Acquisition of >10,000 events was performed.scatter diagram showing peripheral blood lymphocytes stained with SLM30096M. The cells were incubated with the antibody (SLM30096M, 5 µl/Test) for 30 min at 22°C. The secondary antibody used for 40 min at room temperature Acquisition of >10,000 events was performed.
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Unit:
Price: $
Product PDFs
Datasheet:


This gene encodes a member of the immunoglobulin gene superfamily. Expression of this cell surface protein is restricted to B cell lymphocytes. This protein is a reliable marker for pre-B cells but its expression diminishes during terminal B cell differentiation in antibody secreting plasma cells. The protein has two N-terminal extracellular Ig-like domains separated by a non-Ig-like domain, a hydrophobic transmembrane domain, and a large SLCterminal cytoplasmic domain. This protein forms a complex with several membrane proteins including complement receptor type 2 (CD21) and tetraspanin (CD81) and this complex reduces the threshold for antigen-initiated B cell activation. Activation of this B-cell antigen receptor complex activates the phosphatidylinositol 3-kinase signalling pathway and the subsequent release of intracellular stores of calcium ions. This protein is a target of chimeric antigen receptor (CAR) T-cells used in the treatment of lymphoblastic leukemia. Mutations in this gene are associated with the disease common variable immunodeficiency 3 (CVID3) which results in a failure of B-cell differentiation and impaired secretion of immunoglobulins. CVID3 is characterized by hypogammaglobulinemia, an inability to mount an antibody response to antigen, and recurrent bacterial infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020]

Function:
Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Subunit:
Forms a complex with CD21, CD81 and CD225 in the membrane of mature B-cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409. Interacts with LYN.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Post-translational modifications:
Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN.

DISEASE:
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

Similarity:
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
P15391

Gene ID:
930

Database links:

Entrez Gene: 930 Human

Entrez Gene: 12478 Mouse

Entrez Gene: 365367 Rat

Omim: 107265 Human

SwissProt: P15391 Human

SwissProt: P25918 Mouse

Unigene: 652262 Human

Unigene: 472 Mouse



Picture

scatter diagram showing peripheral blood lymphocytes stained with SLM30096M. The cells were incubated with the antibody (SLM30096M, 5 µl/Test) for 30 min at 22°C. The secondary antibody used for 40 min at room temperature Acquisition of >10,000 events was performed.
scatter diagram showing peripheral blood lymphocytes stained with SLM30096M. The cells were incubated with the antibody (SLM30096M, 5 µl/Test) for 30 min at 22°C. The secondary antibody used for 40 min at room temperature Acquisition of >10,000 events was performed.
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